This is an automatically translated article.
Ankylosing spondylitis is a disease related to HLA-B27 antigen located on chromosome 6. HLA 27 test is done to detect HLA-B27 antigen, then the HLA-B27 test index will be positive. is the basis for determining ankylosing spondylitis.1. What is the HLA 27 test?
The HLA 27 test is a test that aims to detect the HLA-B27 antigen that is regulated by genetics. HLA-B27 is an antigen located on chromosome 6 and is associated with ankylosing spondylitis.If the HLA-B27 antigen is found on the chromosome, the HLA-B27 test will be positive and it means that the person has a high chance of having ankylosing spondylitis.
Xét nghiệm HLA 27 là xét nghiệm nhằm mục đích phát hiện kháng nguyên HLA-B27 được quy định bởi gen di truyền
2. In what cases is the HLA 27 test indicated?
The HLA-B27 test is indicated for suspected cases of the following diseases:Ankylosing spondylitis; Reactive arthritis (also known as Reiter's syndrome); Psoriatic arthritis ; Juvenile rheumatoid arthritis; Uveitis . If there are symptoms of the above diseases, the patient should immediately go to the medical facility to be assigned to perform the HLA 27 test as a basis for diagnosing the disease, from which to receive timely treatment advice. Manifestations that the patient may encounter include:
Joint swelling, joint pain, stiffness, limited mobility, especially in the spine, lumbar region, ... Conjunctivitis; Skin damage; Urethritis. Testing for HLA-B27 is especially important if there is a family history of ankylosing spondylitis.
Xét nghiệm HLA-B27 được chỉ định đối với những trường hợp nghi ngờ mắc một số bệnh như viêm khớp vảy nến, viêm cột sống dính khớp,...
3. What types of HLA 27 tests are available, and how are they performed?
The HLA-B27 test for ankylosing spondylitis can be performed by Realtime PCR (real-time polymerase chain reaction) or Flow cytometry, specifically As follows:Real-time PCR technique: The test technician or doctor takes 2ml of whole blood as a specimen and uses EDTA for anticoagulation. Within 24 - 48 hours, HLA 27 samples are stored at a temperature between 2 - 8 degrees Celsius and transported to the place of analysis and testing. Then, the test technician extracted the DNA sample in the patient sample and applied PCR technique to analyze the sample. Using the Taqman hydrolysis probe with fluorescence FAM (helps to detect HLA-B27) and JOE/HEX/Cy3 (helps detect intrinsic disease) to detect HLA-B27 antigen to diagnose spondyloarthritis joint adhesion. Flow cytometry technique: After collecting, preserving and transporting the patient sample to the test site, using the Flow cytometry system to detect HLA-B27 antigen on the surface of white blood cells. Use fluorescence to detect antigens, if there is a fluorescence signal, then the antigen is present and vice versa.
Xét nghiệm HLA-B27 để chẩn đoán bệnh viêm cột sống dính khớp
4. What harm does ankylosing spondylitis cause?
Ankylosing spondylitis is a disease found in young people and men are at higher risk. Since antigens are located on chromosomes that are regulated by genes, this is an inherited disease, which can go up to many generations.The disease greatly affects the daily activities and life of the patient, doing:
Atrophy of the ligaments, calcification of ligaments, endarteritis of the joints and joint capsule. Pain in the joints of the spine restricts movement, and pain in the lower extremities may be accompanied. If not detected and treated promptly, the disease leaves severe sequelae, which is the risk of joint deformity and disability. Not only affecting the joints, ankylosing spondylitis can also cause uveitis in the eyes and other organs. In summary, the HLA-B27 test index is the basis for determining the possibility of ankylosing spondylitis. If the test result is positive, it means that the HLA-B27 antigen is found on the chromosome and the chances of getting the disease are very high.
Currently, to get accurate HLA-B27 test results, patients need to go to a reputable hospital with modern equipment for examination and be appointed by a specialist to do the test. At Vinmec International General Hospital, diagnostic imaging plays an extremely important role, not only having the musculoskeletal system, modern equipment: 6 ultrasound rooms, 4 DR X-ray rooms ( 1 full-axis scanner, 1 intensifier, 1 compositor and 1 mammography machine), 2 DR mobile x-ray machines, 2 receiver multi-row computer tomography rooms (1 128 rows and 1 receiver) 16 rows), 2 rooms for magnetic resonance imaging (1 machine 3 Tesla and 1 machine 1.5 Tesla), 1 room for interventional angiography 2 planes and 1 room to measure bone mineral density....Vinmec is also a gathering place. A team of experienced doctors and nurses will greatly assist in the diagnosis and early detection of abnormal signs of the patient's body. In particular, with a space designed according to 5-star hotel standards, Vinmec ensures to bring the patient the most comfort, friendliness and peace of mind.
Please dial HOTLINE for more information or register for an appointment HERE. Download MyVinmec app to make appointments faster and to manage your bookings easily.
