Currently, infertility due to male causes accounts for 40% of infertility causes. Among them, azoospermia or azoospermia is one of the most common causes.

Currently, there are many different techniques for cryopreservation of sperm, but for sperm samples with extremely poor quality, special preservation measures are needed, both to ensure convenience, so that each time of thawing, there is enough sperm needed for one IVF procedure, and to still have enough frozen sperm to use for the next time without having to thaw it many times.