Real-Time PCR (Polymerase Chain Reaction) testing technique is used to amplify and simultaneously determine the quantity of target DNA molecules. Real-time PCR testing becomes useful for the accurate identification and characterization of pathogens, playing an important role in the treatment of sexually transmitted diseases.

HPV (Human Papilloma virus) is a common sexually transmitted virus, with many different strains. Some strains can lead to cervical cancer, vaginal cancer, genital warts, or anal and penile cancer.