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The method of sperm washing is aimed at removing dead sperm, separating and selecting sperm with good motility from the seminal layer to pump into the uterus. So in infertility treatment techniques, how much sperm concentration after washing is good?1. Overview of sperm washing method
Besides the cause of infertility, the level at different medical centers and the number of follicles of the woman, the quality of the husband's sperm is also one of the factors that determine the success rate. of infertility treatments. Specifically, the higher the motile sperm count, the higher the chances of conception, falling in the general range from 6 to 26%.
The purpose of sperm washing is to remove some semen as well as dead sperm to obtain a sample of many healthy sperm in a small volume. In fact, after releasing into a woman's vagina, semen has many negative effects on sperm, namely:
Semen contains substances that prevent the activation and reaction of sperm heads; Semen contains many bacteria that reside or cause disease on the male genital tract; Prostaglandins present in semen can cause uterine contractions; Dead cells and toxins in semen affect sperm quality as well as normal fertilization physiology; Injecting semen directly into the uterus carries a risk of allergic reactions or anaphylaxis.
Therefore, in addition to increasing the success rate of conception, the technique of separating sperm from semen will largely limit the risk of infection, and at the same time avoid the risk of anaphylaxis that occurs when sperm is injected into the uterus. of the woman (referred to as IUI). In addition, when the sperm has been concentrated to less than 0.5ml, it will not flow back out after IUI operation.
Lọc rửa tinh trùng trước khi làm IUI
The sperm washing method is usually indicated in the following cases:
Injecting sperm into the uterus; Preparing for in vitro fertilization cases ; Infertility of unknown cause.
2. Sperm washing process
2.1. Preparation Performer: Obstetrician-gynecologist; Patient: Abstain from sex for 2-7 days; Equipment and facilities: Centrifuge, microscope, operating cabinet, incubator to keep semen samples; Consumables: Pointed-bottom test tubes, syringes, pipettes, slides and lids, sampling bottles with the patient's name on them and sperm washing media; 2.2. Semen sampling Prepare equipment used to process sperm samples in advance, with the spouse's name or code number; Wash hands and genitals before sampling; The husband can collect sperm by masturbation at the hospital or at home. If collected at home, it must be brought to the hospital within 30-60 minutes after collection; In case of problems, the patient should see a doctor for further advice and guidance.
Mẫu tinh dịch
2.3. Filter and wash sperm. Completely lyse semen in an incubator of 37 degrees C or room temperature for about 30 minutes; Evaluation of semen parameters, including volume, lysis time, pH and sperm concentration; Choose the method of washing according to the concentration scale (gradient) or swimming up (swim-up): The swim-up method: Has the characteristics of supporting good sperm motility, thereby increasing the conception rate. Concentration scale: Usually the sample after filtration is washed with a high total motile sperm count, applicable to many samples, and at the same time removes dirt. Take out the concentrate about 0.3 - 0.5 ml for IUI; Reassess sample motility and sperm concentration after filtration.
3. Evaluation of sperm index after filtering and washing
Indicators used to evaluate before and after washing are usually:
Semen volume; Sperm density; Moving forward; Total motile sperm count. Around the issue of "How much sperm concentration after washing is good?", some studies show that the minimum threshold for motile sperm count after washing to perform injection into the uterus ranges from 800,000 - 20,000,000. Usually, the factors that help increase the probability and success rate of IUI technique are:
The sperm concentration after washing is from 10 million to 20 million; The percentage of motile sperm is over 30%; Total motile sperm count over 5 million. However, there are also cases where the lowest sperm concentration to get pregnant is 7 million, with a cycle pregnancy rate of 13.8%.
4. Note to have a good sperm index after washing and filtering
In order to take care of the fetus in terms of both health and intelligence from the very beginning, in other words, to select high-quality sperm, prospective parents should ensure the condition. the best health during infertility treatment by:
Adhering to a reasonable work and rest regime; Avoid fatigue, control stress (stress); Limit working in an environment where there is contact with toxic substances such as chemicals, pesticides, radioactive substances... to minimize risks; Give up smoking, drinking alcohol or using drugs; Do not wear tight clothing, sit for too long, or soak in hot tubs or saunas; Eating nutritious and safe food helps sperm to be healthy; Prioritize choosing foods rich in folic acid, zinc, vitamin C, cereals, dark green leafy vegetables, meat, seafood, eggs, grapefruit, oranges, lemons, grapes... In short, the sperm washing method helps select healthy, motile spermatozoa and avoid the risk of anaphylaxis occurring when semen is injected into the uterus. The effectiveness of IUI infertility treatment techniques will depend on many factors, including certain requirements on the quality and index of sperm after washing. The concentration of sperm after washing how much is good ranges from 10-20 million.
Trung tâm Hỗ trợ sinh sản - Bệnh viện Vinmec đang thực hiện lọc rửa tinh trùng
The sperm washing method is currently being applied at the Reproductive Center - Vinmec International General Hospital. The method of sperm washing for artificial insemination at Vinmec is carried out methodically and scientifically, in a sterile environment and conditions of international standards, ensuring the best sperm quality. The sperm washing process is performed by leading modern medical equipment, under the supervision of leading infertility treatment experts and doctors, helping the process of artificial insemination. create the best results.
Please dial HOTLINE for more information or register for an appointment HERE. Download MyVinmec app to make appointments faster and to manage your bookings easily.
