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Posted by Dr. Nguyen Van Dinh - Head of Allergy - Clinical Immunology Unit and Doctor Le Duy Cuong, Laboratory - Vinmec Times City International HospitalANA-Hep2 IIFT test is an ANA screening test by Indirect ImmunoFluorescense Technology (Indirect ImmunoFluorescense Technology) using Hep2 (Human Epithelial type 2) cell substrate.
1. What is the ANA – Hep2 IIFT test?
Anti Nuclear Antibody (ANA) appears in the blood due to the autoimmune mechanism, these antibodies will attack the nucleus of cells in the body.
According to the American Rheumatology Association, the ANA-Hep2 IIFT test is the gold standard for ANA screening because it is more sensitive than other methods, detecting 150 autoantigens while other techniques only detect up to 10 antigens.
Mẫu nhuộm miễn dịch huỳnh quang đồng nhất của kháng thể DNA sợi đôi trên các tế bào HEp-20-10
2. When is the ANA – Hep2 IIFT test indicated?
This test is ordered when the patient has the following unexplained symptoms:
Fever, fatigue, headache, continuous dizziness. Numbness in limbs, sensitivity to light, hair loss. Polymyositis, dermatomyositis. Injury to the nervous system, organs of the liver, pancreas, heart, lungs, trachea. Symptoms of rheumatoid arthritis, lupus erythematosus, hepatitis, thyroiditis, idiopathic thrombocytopenia. Raynaud's syndrome.
3. Significance of the ANA-Hep2 IIFT . test
ANA test by Immunofluorescence method has the role of screening for autoimmune antibodies such as: dsDNA, Sm, RNP, SS-A, SS-B, Scl-70, Mi-2,.. These antibodies play a role in the diagnosis of many autoimmune diseases such as systemic lupus erythematosus, scleroderma, autoimmune myositis, primary biliary fibrosis,.... Therefore, the ANA-Hep2 IIFT test has a role. role in the screening and diagnosis of autoimmune diseases.
Unlike ELISA testing, which relies on immunofluorescence staining (ANA phenotype), the clinician can limit circulating autoimmune antibodies in the patient's blood/tissue patient. From there, it helps to orient the diagnosis and save testing costs.
Sensitivity and specificity of ANA –Hep2 IIFT are 100% and >86%, respectively.
Xét nghiệm ANA bằng phương pháp miễn dịch huỳnh quang
4. Notes when taking patient samples
The patient eats normally, rests, and is mentally comfortable. Do not use stimulants such as beer, alcohol, tobacco, coffee...
Patients can take venous blood into test tubes that do not contain anticoagulant or contain heparin anticoagulant, centrifuge to obtain serum. /plasma. The serum/plasma sample is sent to the laboratory for analysis.
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