How does sperm thawing take place?

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Sperm thawing is a technique to thaw testicular tissues containing sperm that have been preserved by cryopreservation, then isolate and select good sperm to participate in insemination and support. reproductive.

Sperm cryopreservation is a method of using liquid nitrogen at a temperature of minus 196 degrees Celsius to create false death, stopping sperm activity to help preserve sperm for a long time without affecting. or alter sperm quality.
In contrast, sperm thawing is a technique that helps bring sperm from the storage temperature of minus 196 degrees Celsius to its original temperature and state so that it can be cultured and used in assisted reproduction. produce .
Frozen sperm has to go through many strict stages, so thawing also requires careful steps.
Sperm defrosting is highly dependent on the amount of ice crystals remaining inside the cell after the cryopreservation period. Extending the defrosting time will cause re-crystallization, which if done in too short a time because water can quickly return to the inside of the cell.

To defrost sperm, you need to prepare:
Cup of warm water 37 degrees C. Tools, filter media to wash thawed sperm samples include: centrifuge, microscope (10 and 40 objective), eyepiece 10, operation cabinet, incubator for semen samples, pointed bottom test tube, syringe, pipette, glass slide, cover glass slide, sample collection bottle with husband and wife's name on it, sperm washing media. After being fully prepared, defrost sperm according to the following steps:
First, remove the frozen sperm sample from liquid nitrogen and then leave it at room temperature for about 1-3 seconds to allow the ice to form. The outer covering is completely melted. Place the tube containing the sperm sample in warm water of 37°C for 10-20 minutes. Evaluation of sperm quality according to the standards of the World Health Organization such as sperm density, lysis time, volume... Filter and wash selected sperm samples. Re-evaluate the quality and motility of sperm after washing and filtering. Inject sperm into the uterus (usually 0.3 - 0.5ml) or use in vitro fertilization.

Sperm thawing is an important technique that determines the effectiveness of both natural conception and in vitro fertilization. Sudden temperature changes during defrosting will greatly affect the quality of sperm, even kill sperm due to improper technique, wrong defrosting temperature, defrosting too much. too fast or too slow... Therefore, sperm freezing and sperm thawing should be done in reputable facilities with adequate facilities according to medical standards.

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